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Synthesis of the Antigens and Preparation of Polyclonal Antibody for Chlorpyrifos

Bai Ying-ying

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Abstract

This study reported that the hapten of chlorpyrifos was synthesized by using chlorpyrifos reacted with 3-marcapropanoic acid.The hapten was conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) with the dicyclohexylcarbodimide method to form artificial immune antigen and coating antigen.The anti-chlorpyrifos polyclonal antibodies with high titer were obtained by using the artificial immune antigen to immune in the rabbits,and the titer is 1:160 000 for antiserum.The standard curves are confirmed by experiments,the real detection limit of the ELISA was 0.5 μg.L-1 with an I50 value of 18.2 μg.L-1 and the linear ranging from 1.8 to 1 000 μg.L-1.

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What this paper is about

This study reported that the hapten of chlorpyrifos was synthesized by using chlorpyrifos reacted with 3-marcapropanoic acid.The hapten was conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) with the dicyclohexylcarbodimide method to form artificial immune antigen and coating antigen.The anti-chlorpyrifos polyclonal antibodies with high titer were obtained by using the artificial immune antigen to immune in the rabbits,and the titer is 1:160 000 for antiserum.The standard curves are confirmed by experiments,the real detection limit of the ELISA was 0.5 μg.L-1 with an I50 value of 18.2 μg.L-1 and the linear ranging from 1.8 to 1 000 μg.L-1.

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Available abstract

This study reported that the hapten of chlorpyrifos was synthesized by using chlorpyrifos reacted with 3-marcapropanoic acid.The hapten was conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) with the dicyclohexylcarbodimide method to form artificial immune antigen and coating antigen.The anti-chlorpyrifos polyclonal antibodies with high titer were obtained by using the artificial immune antigen to immune in the rabbits,and the titer is 1:160 000 for antiserum.The standard curves are confirmed by experiments,the real detection limit of the ELISA was 0.5 μg.L-1 with an I50 value of 18.2 μg.L-1 and the linear ranging from 1.8 to 1 000 μg.L-1.

Key concepts: Hapten, Polyclonal antibodies, Antiserum, Bovine serum albumin, Ovalbumin, Antigen, Titer, Chlorpyrifos

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Synthesis of the Antigens and Preparation of Polyclonal Antibody for Chlorpyrifos — Research Paper | ScholarLens