Influence of p53 gene on the expression of Survivin in gastric carcinoma cell strain BGC823

Objective To detect the expression of Survivin and p53 genes in the gastric cancer cell strain BGC823,and to study how p53 gene influences Survivin gene expression and the presence of possible interacting biological mechanisms between Survivin and p53.Methods RT-PCR,real time quan- titative PCR and Western blot techniques were applied to observe the gene expression and protein produc- tion of both Survivin and p53 in the gastric cancer Cell strain BGC823,respectively.Then the BGC823 cells were transfected with wild-typed-p53 plasmid,pcDNA3.0-rp53,using mock-pcDNA3.0 as a con- trol.All cells were incubated,and real-time PCR and Western blot techniques were applied to examine the levels of Survivin mRNA and protein at the intervals of 8,16,and 24 h.Results The results of RT-PCR and Western Blot revealed that the gastric cancer strain did transcribe and express the Survivin and mu- tant-p53 genes.Those wild-typed-p53-processed BGC825 cells revealed no significant impact on Survivin mRNA and protein levels at 8th h after the transfection,but the mRNA and protein levels began to fall at 16th h.This tendency kept going down after 24 h of the transfection.Conclusion The wild-typed p53 gene can inhibit the transcription and expression of Survivin at 16th h after transfection,which is more apparent after 24 h of transfection.