Effects of As_2O_3 on proliferation in mouse C26 colon carcinoma cells

Objective To study the effects and mechanisms of As2O3 on proliferation in mouse C26 colon carcinoma cells. Methods MTT assay was performed to evaluated the effect of 1,2,4,6,8 μmol/L As2O3 on proliferation in mouse C26 colon carcinoma cells for 24 h,48 h and 72 h. Optical microscope and flow cytometry were employed to determining the apoptosis effect and cell cycle distribution of C26 colon carcinoma cells. Results 1,2,4,6,8 μmol/L As2O3 could inhibit the proliferation of cultured C26 colon carcinoma cells in vitro performed by both MTT assay and cell morphological observation,and the proliferative rate decreased in a dose-dependent and time-dependent manner. In addition,the cell cycle of C26 colon carcinoma cells was arrested at G0 /G1 phase. Conclusions As2O3 inhibit the proliferation of cultured C26 colon carcinoma cells in vitro,the mechanisms of which may be involved in cell apoptosis and arrest the cell cycle.