Effects of bone morphogenetic protein 2 on proliferation and apoptosis of colon carcinoma cell line HT-29 in vitro

Objective To investigate the effects of Bone Morphogenetic Protein 2 on cell growth and apoptosis of colon cancer cells.Methods In vitro cultured human colon carcinoma cells were exposed to rhBMP-2 at different concentration and different treating time,then the growth inhibiting effects of rhBMP-2 on the cells were detected by MTT assay;cell morphology of apoptotic cell was observed with Hoechest 33342 staining;Annexin V/PI staining was applied for apoptosis detected by flow cytometer.Results BMP-2 has the effect of anti-proliferation and inducing apoptosis on colon carcinoma HT-29 cells.The suppression and apoptosis were in concentration-dependent manner.Such effect were showed obviously in group 100ng/ml for 24h.Marked morphological changes of cell apoptosis including condensation of chromatin and nuclear fragmentation were observed clearly by Hoechst 33342 staining after treatment with BMP-2 for 24 h.BMP-2 induced apoptosis of HT-29 for 24h stained with AnnexinV/PI analyzed by flow cytometry also showed the apoptosis induced by BMP-2 had significant differences with control group.Conclusion BMP-2 has the effect of inducing apoptosis and anti-proliferation on colon carcinoma HT-29 cells in vitro at certain concentration and certain period.