Determination of residual ivermectin,avermectin,moxdectin,eprinomectin and doramectin in aquatic products using HPLC with fluorescence detection

A method using high performance liquid chromatography(HPLC) with fluorescence detection was employed for the simultaneous determination of the ivermectin,avermectin,moxdectin,eprinomectin and doramectin in aquatic products.Samples were extracted with isooctane,dissolved with hexane after concentration,re-extracted with acetonitrile and then derivatised with N-methylimidazole and trifluoroacetic anhydride.The target compounds were separated in ODS-3 chromatographic column and detected by high performance liquid chromatography with fluorescence detection.The limit of detection of the five compounds is 1.0 μg/kg.The average recoveries were between 78.9%-106% with relative standard deviation(RSD) 5.8%-11.2% in the spiked concentration range of 1-50 μg/kg.