Chrysin sensitizes apoptosis of human hepatocellular carcinoma(HCC) Bel-7402 cells induced by rhsTRAIL

Objective o investigate whether chrysin(ChR) sensitizes apoptosis induced by recombinant human solubility TNF-related apoptosis-inducing ligand(rhsTRAIL) in human hepatocellular carcinoma(HCC) Bel-7402 cell line.Methods Bel-7402 cell line was cultured in vitro.The cell death rate was determined by flow cytometry using PI fluorescence staining.The characteristic features of cell apoptosis was examined using DNA agarose gel electrophoresis.The expressions of DR5 were analyzed by Western blot.Results The cell death rate in human hepatocellular carcinoma Bel-7402 cell line by ChR(40μmol/L) or rhsTRAIL(100ng/mL) or both for 48h were 4.91%±0.38%,5.89%±0.39% and 28.7%±2.50%,respectively.The ladder band could be shown in DNA agarose gel electrophoresis after treatment with rhsTRAIL(100ng/mL) for 48h pretreated with ChR(40μmol/L) for 30 minute.ChR upregulated expression of DR5 in Bel-7402 cells,in a time-and concentration-dependent manner.Conclusion ChR at suboptimal concentration possess sensitization of rhsTRAIL induced apoptosis of human hepatocellular carcinoma Bel-7402 cells.