Chrysin Enhances rhsTRAIL Induced the Cytotoxicity in Human Gastric Cancer SGC-7901 Cell Line

Objective To investigate whether chrysin(ChR) enhance cytotoxicity induced by recombinant human solubility TNF-related apoptosis-inducing ligand(rhsTRAIL) in human gastric cancer SGC-7901 cell line.Methods Human gastric cancer SGC-7901 cell line was cultured in vitro.The cytotoxicity was determined using MTT assay.The cell death rate was examined by flow cytometry using PI fluorescence staining.Results IC50 of cell viability inhibition in human gastric cancer SGC-7901 cells by ChR and rhsTRAIL alone or in combination of both were 134μmol/L,402ng/mL and 47ng/mL,respectively.The CI value for ChR and rhsTRAIL was 0.4676.The death rate in human gastric cancer SGC-7901 cells by ChR(40μmol/L) or rhsTRAIL(100ng/mL) or both were 4.65%±0.58%,3.60%±0.16% and 49.87%±4.27 %,respectively.Conclusion ChR at suboptimal concentration possess augmentation of rhsTRAIL induced cytotoxicity of in human gastric cancer SGC-7901 cells.