Development of a LUX PCR Assay for Detection of Streptococcus suis Type 2

The infection caused by Streptococcus suis type 2 is an important zoonosis.Based on the conserved cps2J region of Streptococcus suis type 2,the LUX primers were designed and the LUX PCR assay was developed to detect Streptococcus suis type 2.The sensitivity and specificity of the developed assay were analyzed by using the tonsils samples artificially contaminated with plasmid DNA as testing materials.Results showed that the developed Streptococcus suis serotype 2 specific LUX PCR assay was sensitive enough to detect 100 copies of plasmid DNA from the mask samples.The assay could distinguish Streptococcus suis serotype 2 from Streptococcus agalactiae,swine Pasteurellosis mutocida,Streptococcus faecalis,Streptococcus suis equi subsp equi,swine Cholerasuis and other swine bacteria pathogens.The developed assay can serve a candidate for the quick detection of Streptococcus suis serotype 2 infection in the field.