EFFECT OF SODIUM NITRITE ON THE CHROMOSOME ABERRATION IN LUNG CELLS OF CHINESE HAMSTER

[Objective]To explore the effects of Sodium Nitrite(NaNO2)on chromosome aberration in lung cells of Chinese hamster.[Methods]The effect of NaNO2 on the IC50 of CHL cells was determined,these Chinese hamsters were divided into different groups in line with different dosage to perform the official test so that to observe the chromosome aberration status with S9 and without,and the results were determined according to the standardized criteria.[Results]When the concentrations of NaNO2 were 128,12.8 and 1.28 μg/ml without S9,the chromosome aberration rates were at the range of 5% to 10%,which was suspected to be positive. While the Chinese hamsters in group with high concentration of 1.28mg/ml,the chromosome aberration rate was at the range of 10% to 20%,which showed to be positive reaction. When adding S9 after treating with NaNO2,the chromosome aberration rates of CHL cells induced by the dosage groups with the concentration of 12.8 μg/ml and 1.28 μg/ml were at the range of 5% to 10%,which was suspected to be positive. While the chromosome aberration rates in groups at the concentration of 128 μg/ml and 1.28mg/ml were at the range of 10% to 20%,which showed to be positive reaction.[Conclusion]NaNO2 can induce chromosome aberration in CHL cells at the concentration of 1.28mg/ml.