Study on Rapid Detection of Clavibacter michiganensis subsp. michiganensis by Real-time Fluorescent PCR

Specific primers and Taq Man probe,which were designed based on the sequence of ITS gene(16S-23SrDNA spacer region) in Clavibacter michiganensis subsp.michiganensis,were used to detect the standard bacteria from both Clavibacter michiganensis subsp.michiganensis and other germina by using real-time fluorescent PCR assay.The results showed that the PCR product from Clavibacter michiganensis subsp.michiganensis had strong specific fluorescent signal,while that from other germina hadn't,which indicated that the primers and Taq Man probe had the specificity to detect Clavibacter michiganensis subsp.michiganensis.The system could detect the DNA solution of 52.3fg/μL in the gradient dilution test of Clavibacter michiganensis subsp.michiganensis.