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Study on Rapid Identification of Clavibacter michiganensis subsp. michiganensis by Molecular Biological Method

Zheng Yuan

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Abstract

A rapid molecular biological identification method of Clavibacter michiganensis subsp.michiganensis is established by using one pair of primers unique to tomA gene segments.A specific DNA amplicon was present in the standard strains,but absent in the non-standard strains.It showed that this pairs of primers was specific for rapid identification of C.michiganensis subsp.michiganensis.The sensitivity of this method was confirmed by testing the serial dilutions of C.michiganensis subsp.michiganensis,which could be determined at the concentration of 10~6CFU/mL.

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What this paper is about

A rapid molecular biological identification method of Clavibacter michiganensis subsp.michiganensis is established by using one pair of primers unique to tomA gene segments.A specific DNA amplicon was present in the standard strains,but absent in the non-standard strains.It showed that this pairs of primers was specific for rapid identification of C.michiganensis subsp.michiganensis.The sensitivity of this method was confirmed by testing the serial dilutions of C.michiganensis subsp.michiganensis,which could be determined at the concentration of 10~6CFU/mL.

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Available abstract

A rapid molecular biological identification method of Clavibacter michiganensis subsp.michiganensis is established by using one pair of primers unique to tomA gene segments.A specific DNA amplicon was present in the standard strains,but absent in the non-standard strains.It showed that this pairs of primers was specific for rapid identification of C.michiganensis subsp.michiganensis.The sensitivity of this method was confirmed by testing the serial dilutions of C.michiganensis subsp.michiganensis,which could be determined at the concentration of 10~6CFU/mL.

Key concepts: Clavibacter michiganensis, Amplicon, Biology, Serial dilution, Microbiology, Identification (biology), Gene, DNA

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