Simultaneous determination of ephedrine hydrochloride and amygdalin in Xiao′erfeirekechuan effervescent granules by RP-HPLC

Objective To establish an RP-HPLC method for simultaneous determination for ephedrine hydrochloride and amygdalin in Xiao′erfeirekechuan(traditional Chinese medicine)effervescent granules.Methods The column of Kromasil ODS column(250 mm×4.6 mm,5 μm)was used,the mobile phase was acetonitrile-water(40 mmol·L-1 phosphonic acid)-triethylamine(V∶V∶V=10∶90∶0.1)and detection wavelength was set at 206 nm.Results The linear range of ephedrine hydrochloride was 25-300 ng,r=0.999 8,amygdalin was 0.5-10 μg,r=0.999 4.The average recoveries of ephedrine hydrochloride and amygdalin were 98.7% with RSD 1.72%,99.8% with RSD 1.09%.The determination results of ephedrine hydrochloride and amygdalin were 1.728 mg·bag-1 and 32.07 mg·bag-1.Conclusions The developed method can be used to determine the ephedrine hydrochloride and amygdalin in Xiao′erfeirekechuan effervescent granules.