Effect of electroacupuncture with different acupoint group on IL-1β,TNF-α and IL-6 in the hypothalamus of insomnia rats

Aim:To observe the effects of Electroacupuncture at different acupoints group on the content of Interleukin-1β(IL-1β),Tumor Necrosis Factor-α(TNF-α),Interleukin 6(IL-6) in the hypothalamus of insomnia rats to investigate the mechanism of Electroacupuncture treatment of insomnia. Methods: Thirty-six SD rats were randomly divided into six groups: control group,model group,diazepam group,Electroacupuncture group I(Baihui(DU20),Shendao(DU11),Xinshu(BL15)),Electroacupuncture group II(Sanyinjiao(SP6),Shenmen(HT7)),Electroacupuncture group III(Shenmai(BL62)、Zhaohai(KI6)).The model of insomnia rats were established by DL-P-Chlorophenylalanine(PCPA)peritoneal injection.The diazepam group rats were treated by diazepam peritoneal injection.Each Electroacupuncture group rats were treated by Electroacupuncture at different acupoints.Cytokines such as IL-1β,TNF-α,IL-6 in the hypothalamus of insomnia rats in each group were determined after 6 days continuously treatment.Results: After Electroacupuncture treatment the content of IL-1β,TNF-α and IL-6 increased obviously in contrast with model group and diazepam group(P0.05,P0.01).Among the three Electroacupuncture groups,groupII was better than the group I(P0.05),but there was no significant difference between the group III and the other two group(P0.05).Conclusion:The mechanism of Electroacupuncture treatment of insomnia may be due to the increase of cytokines such as IL-1β,TNF-α,IL-6 in the hypothalamus of insomnia rats.Electroacupuncture group II is better than group I and it may be suggest that Sanyinjiao(SP6),Shenmen(HT7) is better than other acupoints in treating insomnia.