PREPARATION OF FUMONISIN B_1 ARTIFICIAL ANTIGEN AND MOUSE POLYCLONAL ANTISERUM

To prepare high sensibility and specificity polyclonal antiserum against FB1,Fumonisin B1(FB1) artificial antigens were synthesized and identified through animal immune in this study.FB1 were separately coupled with carrier protein BSA and OVA using EDC method,then the immune antigen FB1-BSA and detection antigen FB1-OVA were obtained.The BALB/c mice were respectively immune at the dose of 10 and 30μg,every four weeks,four times in total.At the thirty days after the last immunity,the poly-antiserum was prepared through broken tail method.The titer of antiserum was determined by indirect ELISA,sensibility and specificity was determined by indirect competitive ELISA,respectively.The results showed that the high titer of the antiserum of six mice were above 1∶104.The antiserum of No.3 mouse has the best sensibility.Its IC50 was 61.3ng/ml and has good specificity.In this study,FB1 artificial antigens were successful synthesized.FB1 poly-antiserum with high sensibility and specificity were obtained,and this poly-antiserum may lay a foundation for the further study about FB1 monoclonal antibody preparation and the immunology fast detection methods.