Expression of Rb,E2F-1 and P21 protein in bladder tumor and their pathological significance

Objective: To detect the expression of Rb,E2F-1 and P21 in bladder tumor and explore their role in the development of bladder tumor and clinicopathological significance. Methods: Rb,E2F-1 and P21 protein were detected by immunohistochemical method in 122 cases of bladder tumor. Results: The positive expression rate of Rb protein in bladder transitional cell papilloma and bladder cancer were 93.3%(28/30),68.5%(63/92),the difference was significant (P=0.007).The positive rates of E2F-1 and P21 in bladder cancer were 70.7%(65/92),72.8%(67/92),which was significantly higher than 24% and 22.8%in bladder papillary epithelioma(P0.05,P0.05).Rb negatively correlated with E2F-1(r= -1.000,P0.05)and P21(r=-1.000,P0.05);E2F-1 positively correlated with P21 (r=1.000,P0.05). Conclusion: The down-regulation of Rb expression and up-regulation of E2F-1 and P21 expression may contribute to the carcinogenesis and development of bladder cancer and can be a biological marker for diagnosis and judging malignancy degree of bladder cancer.