Establishment of Human Laryngeal Cancer Cell Line Transfected with Human IL-24 cDNA and Its Expression

Objective To establish human laryngeal cancer cell line transfected with human IL-24 cDNA and demonstrate its expression in laryngeal cancer cell.Methods The recombinant expression plasmid pcDNA3.1(+)-hIL-24 was transfected into human laryngeal cancer cell line Hep-2 cells and the positive clone was screened by G418.Then the expression of hIL-24 in Hep-2 cells was tested by RT-PCR and Western blot assay;the secretion of hIL-24 in medium was detected by ELISA.Results Human IL-24 cDNA was successfully integrated into Hep-2 cells and overexpressed.The result of RT-PCR showed that IL-24 mRNA was detected.The concentration of hIL-24 in the supernatants of the 106 transfected Hep-2 cell cultures in 24 hours as detected by ELISA was(162.8±15.4)pg/mL,no hIL-24 expression was detected in control group.Conclusion Human IL-24 cDNA was successfully integrated into Hep-2 cells and could be expressed with high efficiency.