Establishment of Human Laryngeal Cancer Cell Line Transfected With Human IL-15 cDNA and Its Expression

Objective To establish the human laryngeal cancer cell line transfected with human IL-15 cDNA and its expression in tranfected laryngeal cancer cell was evaluated. Methods The recombinant expression plasmid pcDNA3.1 (+)-hIL-15 was transfected into human laryngeal cancer cell line Hep-2 cells and the positive clone was screened by G418. The expression of hIL-15 in Hep-2 cells was tested by both RT-PCR and Western blot assay; and the secretion of hIL-15 in the medium was detected by ELISA. Results The human IL-15 cDNA was successfully integrated into Hep-2 cells and overexpressed. RT-PCR results showed positive hIL-15 mRNA. The concentration of hIL-15 in the supernatants of the 106 transfected Hep-2 cell cultures after 24 hours detected by ELISA was 185.0±12.2 pg/ml; in contrast, no hIL-15 expression was detected in the control. Conclusion Human IL-15 cDNA is successfully integrated into the Hep-2 cells and expresses with high efficiency demonstration.