Establishment of a Multi polymerase Chain Reaction (multi PCR) for Detecting Mycoplasma gallisepticum and Mycoplasma synoviae

On the basis of the gene library bank of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) the two pairs of primers that are complementary to a given gene sequence of MG and MS were designed and synthesized, with which a multi PCR was performed for detecting MG and MS, when the objective template DNAs of MG and MS existed in samples the PCR amplified two bands the sizes of which are as same as bands experimentally designed as one 732 bp (MG) and as another one 207 bp (MS) would be obtained. However, amplification of other different eight pathogens of avian diseases all were negative. The sensitivity test revealed that 100 fg of DNA of MG and MS can be detected with the multi PCR.