Establishment of PCR for Detection of Mycoplasma gallisepticum

A pair of primers was designed based on fMG-2 sequences of avian Mycoplasma.The primers selectively amplified a 726 bp product from Mycoplasma gallisepticum(MG),but did not amplify the DNA or RNA of other bacteria or viruses.The detection threshold of PCR for MG was 3 pg.The samples detected by PCR,and isolation and identification.Using PCR,20.5% of field samples was positively detected,it was higher than that of isolation and identification.The results indicated that this technique is a useful screening assay for the detection of MG.