Inhibition of small interference RNA targeting FOXO3a on expression of FOXO3a in HepG2

Objective To explore the effects of FOXO3a siRNAs on FOXO3a expression,cell cirele and apoptosis of HepG2 cells.Methods Three FOXO3a specific siRNAs were selected with the suppressing effect. RNAi expression vectors were constructed and transfected into HepG2 cells.RT-PCR and Western Blot were used to detect the expression of FOXO3a.The proliferation of the cells was measured by MTT test.The apoptosis of HepG2 cells was measured by flow cytometry.A nonsense small RNA was set as negative control. Results There were the export of FoxO3a to the cytoplasm from nucleus in P2,P3 group. When compared with the control groups,the FOXO3a-specific siRNAs inhibited the expression of FOXO3a in the protein and mRNA level, and suppressed the proliferation and induced the apoptosis of the HepG2 cells(P0.05).Conclusion Knockdown expression of FOXO3a can decrease apoptosis and increase proliferation of HepG2 tumor cell,and FOXO3a gene can be a powerful target gene in treating liver cancer.