Effects of siRNA enkaryotic expression vectors targeting PEG10 gene on cell cycle in HepG2 cells

Objective To investigate the effects of siRNA eukaryotic expression vectors targeting PEG10 on HepG2 cell cycle and cell-cycle regulatory proteins. Methods The psiRNA-PEG10 plasmid was transiently transfected into HepG2 cell with Lipofectamine 2000. The expression of PEG10 in HepG2 was detected by RT-PCR. The effect of PEG10 on the cell cycle was observed by flow cytometry. The mRNA and protein expression of Cyclin D1and p27 were detected by RT-PCR and Western Blotting respectively. Results There was a depressed expression of PEG10 in the cells transfected with psiRNA-PEG10. Upon flow cytometry, there were more cells in G0/G1 phase and fewer cells in G2/M phase in psiRNA-PEG10-transfected cells. The mRNA and protein expression of Cyclin D1 decreased in the psiRNA-PEG10-transfected cells, while p27 expression increased (P0.01). Conclusion The result indicated that PEG10 might play an important role in the pathogenesis of HCC by inhibiting the cell cycle, which was related to the decreased Cyclin D1 and increased p27 expression. SiRNA eukaryotic expression vector targeting PEG10 may become a promising-treatment strategy of HCC.