Determination of sperm testosterone and the observation of testis and epididymis following vasectomy

Objective:To provide some groundwork for the estimation of the ligation of vas deferens as a contraceptive method.Methods: We established the animal models by 40 sexually mature Wistar rats [5(control group)+5(surgery group)]×4, and measured their serum testosterone(ST) levels. We observed the histological changes of their testis and epididymis following the vasectomies in 1, 2, 3, 4 months respectively. Results: No significant difference was found among the compared groups in all experimental intervals; most of all the vasectomized rats showed none infected inflammation both in their testis and epididymis the seminiferous tubules deteriorated and the ductus epididym is dilated; but no anticipated sperm granuloma was found. Conclusion:The ligation of vas deferens can lead to some influnce in the vasectomized animals during a short time limit, and its distinct long term effects need furthermore study.