Influnce of fastigial nucleus electric stimulation on synaptic ultrastructure and synaptophysin expression in hippocampus of neonatal rat

Objective To observe the Influnce of electric stinulation on synaptic ultrastructure and the synaptophysin expression(p38)in hippocampal CA1 area after hypoxia-ischemic brain damage(HIBD)in neonatal rats.Methods The rat model of HIBD was made by the method of Rice in 120 SD rats,and divided randomly into 2 groups:the electric stinulation group,model group.Another 60 rats underwent sham-operation as control.According to the time of 3 day,7day,14day,21day,the three groups were divided randomly into 4 groups.From the second day after HIBD,the rats of the electric stinulation group were stimulated.The hippocampal ultrastructure was observed with electron microscope and the p38 expression was detected immunohistochemically at 3 day,7day,14day,21day in rats.Results The ultrastructure of hippocampus CA1 neuron in the model group were distorted as compared with that in the electric stinulation group and control group.The corrected opticaldensities(COD)of immunoreactive products of the hippocampal p38 were significantly decreased in model group as compared with electric stinulation group at 14d,21d(P0.05).There were no marked difference of immunohistochemically reactived COD of p38 among the three groups at 3d.(P0.05).Conclusions Electric stinulation could improve the synaptic reconstruction and reduce the ultrastructural damage induced by hypoxic-ischemia.