Cytometric changes of living pyramidal neurons in the hippocampal CA1 in neonate rats with hypoxic-ischemic brain damage after intervention of selective COX-2 inhibitor NS398

Objective To study the cytometric changes of living pyramidal neurons in the hippocampal CA1 in neonate rats with hypoxic-ischemic brain damage(HIBD)after intervention of selective COX-2 inhibitor NS398.Methods NS398(20 mg/kg)was intraperitoneally injected 30 min before the model of HIBD Wistar rats was established.Hematoxylin-eosin(HE)staining technique and light-microscopy examination were adopted to count the living pyramidal neurons in the ipsilateral hippocampal CA1 at day 7 after the onset of hypoxic-ischemic damage.HIBD group and control group also examined in the same way.Results The HIBD group displayed a pronounced reduction in living pyramidal neurons in the hippocampal CA1 compared with normal control group(P0.01).NS398 intervention group also displayed a significant CA1 neuron number reduction,but the extent of CA1 neuronal number reduction was significantly less than that of the HIBD group(P0.01).Conclusion Selective COX-2 inhibitor NS398 can attenuate the depletion of pyramidal neurons in the hippocampal CA1 in neonate rats with hypoxic-ischemic brain damage.