Plant regeneration from mature embryo of Buffalograss and its influencing factors

The factors influencing callus induction, plant regeneration from mature embryo of buffalograss and the explant sterilization were studied. The optimal method to obtain the sterile explant is that mature embryo was dipped in 70% ethanol for 60 s, then it was soaked in 75% diluent of NaClO for 30 min, in which 7%~10% chlorine is availed. The callus was induced easily in Murashige and Skoog (1962) (MS) basal medium supplemented with 1.5~3 mg/L 2,4-D. And the callus induction rate reaches to 80.27% with 3 mg/L 2,4-D, but the callus structure is loose. While the quality of the callus cultured in the induction medium with 5 mg/L silver nitrate (AgNO 3) or silver thiosulfate (STS) is better than others. The ingredients in the subculture medium such as three-quarter dosage of macro elements of MS, Polyvinylpyrrol idone (PVP)200 mg/L, vitamin C(Vc) 200 mg/L as well as CH 1000 mg/L mitigated the degree of callus browning, although no significant difference was detected. Plant regeneration of buffalograss was obtained from the callus initiated on the medium containing STS, and the regeneration rate reaches to 10%.