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Study on Spectrophotometric Determination of Iron in Tobacco with Rutin

Hai Huang

Abstract

The color reaction of rutin with iron(Ⅲ) was studied.In the presence of hydrochloric acid solution,rutin can reacts with iron(Ⅲ)to form a stable 3∶1 complex.The malor absorptivity is 1.38×10 4 L/mol·cm at 590 nm.Beer's law is obeyed in range of 0～50 μg/25 mL.This method can be applied to the determination of iron in tobacco with satisfactory results.

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Key concepts: Rutin, Chemistry, Hydrochloric acid, Molar absorptivity, Nuclear chemistry, Spectrophotometry, Color reaction, Chromatography