2006•Food ScienceRequires access

Study on Protoplast Formation and Regeneration of Pectinase Producing Bacterial ZH-g

Mingyan Yang

Abstract

The optimum conditions for preparation and regeneration of ZH-g protoplast were obtained after studying the effects of different factors on them.Under this circumstance-treating strains in log phase with concentration of 1.2mg/ml lysozyme for 2 hours,after pretreatment on the cells with 0.6μg/ml ampicillin,the protoplast formation is up to 92%,and regeneration reaches 50.7%.

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Available abstract

Key concepts: Protoplast, Lysozyme, Pectinase, Regeneration (biology), Chemistry, Microbiology, Biology, Enzyme