Protective effect of ischemic postconditioning on lipid peroxidation of lung following intestinal ischemia reperfusion in rats

Objective: To investigate the protective effect of ischemic postconditioning(I-post C) on lipid peroxidation of lung following intestinal ischemia reperfusion(I/R) in rats.Methods: Thirty-two male Wistar rats were randomly divided into sham,I/R,intestinal ischemic postconditioning(II-post C) and limb ischemic postconditioning(LI-post C) groups.Model of intestinal I/R injury was made by clamping super mesenteric artery for 45 min followed by 120 min of reperfusion in rats.At the end of the experiment,the changes of arterial blood gas level and lung index were measured and the morphological changes of lung tissue were observed through light microscope.Superoxide dismutase(SOD) activity and malondialehyde(MDA) content in plasma and lung tissue were detected.Results: II-post C and LI-post C improved the lung respiratory function,charactered by the increase of PaO2 and decrease of PaCO2(P0.05 vs I/R group).The lung index was decreased(P0.01),and the pathologic lesion of lung tissue was alleviated significantly by II-post C and LI-post C in comparison with those in I/R group.In addition,both II-post C and LI-post C markedly inhibited the decrease of SOD activity and increase of MDA content in plasma and lung tissue(P0.05 or P0.01) induced by intestinal I/R.Conclusion: I-post C attenuates lung injury induced by intestinal ischemia reperfusion in rats,which is related to the inhibition of lipid peroxidation reaction.