Effect of ischemic preconditioning on lipid peroxidation of lung reperfusion injury

AIM: To evaluate the effect of ischemic preconditioning (IP)on lipid peroxidation of lung reperfusion injury in situ of rabbits . METHODS: Left lung ischemia was created by occluding left hilum. At the end of experment, lung tissue was excised for measurement of superoxide dismutase(SOD)and glutathione peroxidase (GSHpx) activities by direct method of DTNB, and malondialdehyde (MDA) content by the barbituric acid method. Histolgic change of lung injury was assessed by quantitative method. RESULTS: The MDA content of left lung was increased and SOD, GSHpx activities decreased significantly in ischemia/reperfusion group (I/R group) compared with sham group and ischemia alone group (P 0.01, respectively); Severe histologic injury also occurred in I/R group. There are lower MDA content and higher SOD and GSHpx activities with less lung injury in IP + I/R group than in I/R group (P 0.05). CONCLUSION Ischemic preconditioning attenuate lipid peroxidation induced by nomothermic ischemia reperfusion injury in rabbit lung.