Construction of Hepatitis B Virus X Gene Eukaryotic Expression Vector

Objective:To construct a highly effective eukaryotic expression vector pCI-neo with HBV X gene(pCI-neo-X).Methods:HBV DNA was extracted from the HBV DNA positive serum.HBV X gene was amplified by PCR,and identified by gelose electrophoresis analysis.The PCR products and the eukaryotic expression vectors pCI-neo were digested by restriction endonucleases,then the digested X gene was inserted into the digested eukaryotic expression vector.After screening,the positive recombinants were picked out.Results:PCR analysis showed that the HBV X gene was successfully inserted into pCI-neo vector.Conclusion:The construction of the eukaryotic expression vector pCI-neo will lay solid foundations for further study of HBVX gene.