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2005•Chinese journal of integrated traditional and Western medicineRequires access

Construction of Two Eukaryotic Expression Vectors of HBV X Gene with Different Selection Characteristics

He Xing, Yang Xu, Lei Jian-hu

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Abstract

Objective:To construct two eukaryotic expression vectors of HBV X gene with different selection characteristics.Methods:X gene from plasmid pEcob6 was amplified by PCR assay with specific primers.With the characteristics of multiple clone sites in pCEP4 and pcDNA3.1(+),pGEM~(○R)-T Easy Vector was selected to construct intermediate vector pEasy-X.After enzyme digestion,specific fragments were linked to construct pCEP4-X and pcDNA3.1(+)-X vectors.Results:X gene was succeesfully cloned from pEcob6 by PCR assay and the amplified gene products were successfully inserted into pEasy-X vectors.The successful insertion of HBV X gene into pCEP4-X and pcDNA3.1(+)-X vectors was confirmed by enzyme digestion,PCR assay and sequencing.Conclusion:Two eukaryotic expression vectors of HBV X gene with different selection characteristics were successfully constructed.

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What this paper is about

Objective:To construct two eukaryotic expression vectors of HBV X gene with different selection characteristics.Methods:X gene from plasmid pEcob6 was amplified by PCR assay with specific primers.With the characteristics of multiple clone sites in pCEP4 and pcDNA3.1(+),pGEM~(○R)-T Easy Vector was selected to construct intermediate vector pEasy-X.After enzyme digestion,specific fragments were linked to construct pCEP4-X and pcDNA3.1(+)-X vectors.Results:X gene was succeesfully cloned from pEcob6 by PCR assay and the amplified gene products were successfully inserted into pEasy-X vectors.The successful insertion of HBV X gene into pCEP4-X and pcDNA3.1(+)-X vectors was confirmed by enzyme digestion,PCR assay and sequencing.Conclusion:Two eukaryotic expression vectors of HBV X gene with different selection characteristics were successfully constructed.

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Available abstract

Objective:To construct two eukaryotic expression vectors of HBV X gene with different selection characteristics.Methods:X gene from plasmid pEcob6 was amplified by PCR assay with specific primers.With the characteristics of multiple clone sites in pCEP4 and pcDNA3.1(+),pGEM~(○R)-T Easy Vector was selected to construct intermediate vector pEasy-X.After enzyme digestion,specific fragments were linked to construct pCEP4-X and pcDNA3.1(+)-X vectors.Results:X gene was succeesfully cloned from pEcob6 by PCR assay and the amplified gene products were successfully inserted into pEasy-X vectors.The successful insertion of HBV X gene into pCEP4-X and pcDNA3.1(+)-X vectors was confirmed by enzyme digestion,PCR assay and sequencing.Conclusion:Two eukaryotic expression vectors of HBV X gene with different selection characteristics were successfully constructed.

Key concepts: Gene, Plasmid, Vector (molecular biology), Biology, Molecular biology, clone (Java method), Selection (genetic algorithm), Expression vector

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