Expression and Pathological Meaning of COX-2,PCNA,VEGF and MVD in Non-small Cell Lung Cancer

Objective To investigate the expression of cyclooxygenase 2(COX-2),proliferating cell nuclear antigen(PCNA),vascular endothelial growth factor(VEGF) and microvessel density(MVD) in non-small cell lung cancer(NSCLC) and their pathological meaning.Methods Immunohistochemical staining(S-P method) for COX-2,PCNA,VEGF and CD34 was performed in 78 cases with NSCLC;the MVD was marked by CD34.Results The positive rate of COX-2,PCNA and VEGF were 61.5%,89.74% and 60.25% respectively.The value of MVD marked with CD34 was(38.50±7.59).The expression of COX-2 and PCNA was positively correlated with histological stage,clinical stage and lymph node metastasis of NSCLC(P0.01).The positive rate of PCNA expression was significantly higher in patients of clinical stage Ⅲ～Ⅳ than in those of stage Ⅰ～Ⅱ(P0.01).The positive rate of VEGF expression was significantly higher in patients with lymph node metastasis than in those without lymph node metastasis(P0.01).There was a significant positive correlation between the expression of COX-2 and VEGF.The MVD in positive COX-2 group was significantly higher than that in negative group.The positive grades of COX-2 was closely associated with expression of PCNA protein.Conclusion COX-2,PCNA,VEGF and MVD play cooperate roles in lung cancer development and can be utilized as a useful predictor of prognostic evaluation for NSCLC patients.