~(99)Tc-MDP on aqueous humor and serum cytokines of experimental uveitis in rabbits

Objective To investigate the therapeutic effects of 99Tc-MDP on the experimental uveitis in rabbits.Methods Twenty-one experimental rabbits were randomly divided into normal sodium control group,dexamethasone administration group and 99Tc-MDP administration group,7 rabbits in each group.ELISA assay was applied to detect the levels of the interleukin-10(IL-10)and transforming growth factor-β(TGF-β)in the aqueous humor,the levels of tumor necrosis factor-α(TNF-α)and interferon γ(IFN-γ)in serum before establishing the models,at 5 days after establishing the models,at 10 days after drug administration and at 5 days after stopping administration.Results The levels of IL-10 and TGF-β in aqueous humor of normal sodium control group were(86.2±51.2)ng·L-1 and(91.2±67.9)ng·L-1 at 10 days after drug administration lower than(95.1±44.7)ng·L-1 and(135.6±106.3)ng·L-1 at 5 days after establishing the models,respectively.The levels of TNF-α and IFN-γ in serum of normal sodium control group were(151.9±57.9)ng·L-1 and(4.7±1.2)ng·L-1at 10 days after drug administration lower than(175.6±96.0)ng·L-1 and(5.8±2.3)ng·L-1 at 5 days after establishing the models,respectively.The levels of IL-10 and TGF-β in aqueous humor of dexamethasone administration group were(106.9±28.5)ng·L-1 and(168.1±56.6)ng·L-1 at 10 days after drug administration higher than(82.7±41.4)ng·L-1 and(117.4±45.5)ng·L-1 at 5 days after establishing the models,respectively.The levels of TNF-α and IFN-γ in serum of dexamethasone administration group were(80.3±23.4)ng·L-1 and(3.2±2.3)ng·L-1 at 10 days after drug administration lower than(188.2±104.9)ng·L-1 and(5.7±3.6)ng·L-1 at 5 days after establishing the models,respectively.The levels of IL-10 and TGF-β in aqueous humor of 99Tc-MDP administration group were(136.2±47.2)ng·L-1 and(183.1±79.6)ng·L-1 at 10 days after drug administration higher than(98.1±31.3)ng·L-1 and(131.7±53.4)ng·L-1 at 5 days after establishing the models,respectively.The levels of TNF-α and IFN-γ in serum of 99Tc-MDP administration group were(82.7±66.1)ng·L-1 and(2.8±1.4)ng·L-1 at 10 days after drug administration lower than(191.7±72.4)ng·L-1 and(6.8±4.1)ng·L-1 at 5 days after establishing the models,respectively.Compared with the normal sodium control group,both dexamethasone and 99Tc-MDP administration groups could increase levels of IL-10 and TGF-β in aqueous humor,while decrease levels of TNF-α and IFN-γ in serum,and the differences were significant(all P0.05).But there was no difference between dexamethasone administration group and 99Tc-MDP administration group.Conclusions 99Tc-MDP can increase levels of IL-10 and TGF-β in aqueous humor,while decrease levels of TNF-α and IFN-γ in serum of rabbits with experimental uveitis.For the seldom systemic complications,99Tc-MDP has the possibility to be the perspective medicine for uveitis.