Expression of cytokine in aqueous humor of rabbits with traumatic endophthalmitis

Objective To investigate pathogenesis of endophthalmitis by analyzing the concentrations of interferon-γ(IFN-γ),interleukin-6(IL-6),IL-8 and transforming growth factor-β(TGF-β) in aqueous humors of rabbits with traumatic endophthalmitis.Methods A total of 18 rabbits were divided into three groups,6 rabbits in each group.Group Ⅰ was the normal group,and the animal models of traumatic endophthalmitis were established in rabbits of group Ⅱ and Ⅲ.And staphylococcus aureus were injected into the vitreous cavity of traumatic eyes in group Ⅲ.ELISA assay was applied to detect the levels of IFN-γ,IL-6,IL-8 and TGF-β in the aqueous humor before modeling and 6 hours,12 hours,24 hours,48 hours and 96 hours after modeling.Clinical observation was done at 6 hours,12 hours,24 hours,48 hours,96 hours,8 days,16 days after modeling by slit lamp and indirect ophthalmofundoscope and evaluated with clinical grading system.Results The inflammation in group Ⅱ was markedly lighter than that in group Ⅲ,there were statistical differences in clinical inflammatory scores at each time point after 12 hours of modeling between group Ⅱ and group Ⅲ(both P0.05).The levels of IFN-γ in the aqueous humor after modeling in group Ⅱ and Ⅲ all increased,reached the peak at 24 hours after modeling,which were(516.45±20.80)ng·L-1 and(508.21±31.45)ng·L-1,markedly higher than that before modeling(337.15±17.25)ng·L-1,there were statistical differences(both P0.05).The levels of IL-6 in the aqueous humor at 24 hours after modeling in group Ⅱ and Ⅲ reached their peak,which were(61.69±2.11)ng·L-1 and(61.81±2.18)ng·L-1,markedly higher than that before modeling(46.34±2.09)ng·L-1,there were statistical differences(both P0.05).The levels of IL-8 in the aqueous humor at 48 hours after modeling in group Ⅱ and Ⅲ reached their peak,which were(66.78±2.00)ng·L-1 and(64.94±3.39)ng·L-1,markedly higher than that before modeling(44.50±1.40)ng·L-1,there were statistical differences(both P0.05).The levels of TGF-β in the aqueous humor at 24 hours after modeling in group Ⅱ and Ⅲ were the lowest,which were(288.79±7.17)ng·L-1 and(293.99±1.93)ng·L-1,markedly lower than that before modeling(404.45±2.86)ng·L-1,there were statistical differences(both P0.05).Conclusion Traumatic endophthalmitis led to destruction of body's immune system,and the expression of cytokine in the aqueous humor are changed,so some intervene measures should be used to control the inflammation at early stage after injury.