2005•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access
Influence of transection of cervical sympathetic trunck on activity of NOS and content of estrin in rats with pregnancy-induced hypertension syndrome
Lihong Zhang, Guo‐Fu Li, Jianjun Cui
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Abstract
[Objective] To observe the influence of transection of the cervical sympathetic track(TCST)on the activity of NOS and the content of estrin in the rats with pregnancy-induced hypertension syndrome(PIH).[Methods] Pregnant Wistar rats were randomly divided into 5 groups.Control group(A):Saline was injected subcutaneously from the 14th day to 20th day of gestation.PIH group1(B1)and group2(B2):L-NAME was respectively injected with 12.5 mg/100 g and 6.25 mg/100 g every day,then the other procedures were the same as group A.Operation group(C):TCST was operated on the 14th day of the gestation,then the other procedures were the same as group B1.Sham operation group(D):The cervical sympathetic trunk was only separated and exposed on the 14th day of the gestation,then the other procedures were the same as group B1.The changes of pregnant rats and their pups were observed.The NOS activity and the content of estrin in the blood serum of pregnant rats were measured.[Results] Except base value of the BP and protein in urine of the pregnant rats,they in Group B1,B2 were higher than those in Group A significantly(P 0.01),and in Group B2 were lower than those in Group B1 markedly(P 0.01).There was no marked difference between GroupC and Group A(P 0.05).In comparrsion with those in Group A,the size and body weigh of fetus in Group B1,B2 decreased markedly(P 0.01).The above indexes in Group B1 were lower than those in Group B2 markedly(P 0.01,0.05).The changes of the rate of embryo absorption and fetal death,and deformity rate of the fetal rats were contrary to the above indexes.Significant difference was not found between GroupC and Group A(P 0.05).The NOS activity and the content of estrin in the blood serum in Group B1,B2 were lower than those in Group A markedly(P 0.01).Those in GroupB1 were lower than those in Group B2 obviously(P 0.01,0.05).Those in Group C were higher than those in Group B1 markedly(P 0.01),and there was no significant difference compared to Group A(P 0.05).[Conclusion] TCST make pregnant rats avert the effect of PIH,and it can be related to the improving of the NOS activity and the content of estrin in the blood serum.