Comparison of specificity and sensibility of three methods of examination of antibody against syphilis

Objective: To compare the specificity and sensibility of examination of antibody against syphilis with TRUST,ELISA and TPHA,and to increase the security of blood transfusion.Methods: 7733 blood samples from the patients were collected before these patients received the blood transfusion and the antibody against syphilis was determined.3104 of 7733 ones were examined with TRUST and the other 4629 ones with ELISA.Among these 4629 ones,98 ones with OD values close to cut off were determined with double-hole ELISA and TPHA.Results: The positive rate of antibody against syphilis was 1.13% with TRUST and 2.68% with ELISA.Between TRUST and ELISA,the difference of the positive rate of the antibody against syphilis was very significant(P0.01),and between ELISA and TPHA,the difference of the positive rate was also significant(P0.05).Conclusion: ELISA is more sensitive than TRUST.Compared with TPHA,ELISA is more sensitive,but less specific.AAbstract:Objective:To compare the specificity and sensibility of examination of antibody against syphilis with TRUST,ELISA and TPHA,and to increase the security of blood transfusion.Methods:7733 blood samples from the patients were collected before these patients received the blood transfusion and the antibody against syphilis was determined.3104 of 7733 ones were examined with TRUST and the other 4629 ones with ELISA.Among these 4629 ones,98 ones with OD values close to cut off were determined with double-hole ELISA and TPHA.Results:The positive rate of antibody against syphilis was 1.13% with TRUST and 2.68% with ELISA.Between TRUST and ELISA,the difference of the positive rate of the antibody against syphilis was very significant(P 0.01),and between ELISA and TPHA,the difference of the positive rate was also significant(P 0.05).Conclusion:ELISA is more sensitive than TRUST.Compared with TPHA,ELISA is more sensitive,but less specific.