The Optimization of in Vitro Culture Conditions of Blastocystis hominis in RPMI 1640 Mediums

Objective To optimize in vitro culture conditions of Blastocystis hominis in RPMI1640 medium. Method The acidity, inoculation-dose, sorts and concentration of serum, temperature, oxygen, and selection of stool specimens were studied. Result pH 7.5,2×105 cell/tube,20% bovine serum,1×105 IU penicillin and 1.6×105 IU streptomycin,37℃ are the best in vitro culture conditions for Blastocystis hominis. Conclusion Mucous and bloody stool specimens that contained mainly granular forms is a better source of Blastocystis hominis for the culture. The optimal condition for culturing Blastocystis hominis is in RPMI1640 at pH7.5 with 20% calf serum, streptomycin, and penicillin, under anaerobic condition at 37℃. Blastocystis hominis can culture for long time with subculture at every 6 days.