Experimental observation of Blastocystis hominis cultured in Modified RPMI 1640

Objective: To study the effect of modified RPMI1640 in culturing Blastocystis hominis.Methods: Trophozoites of Blastocystis hominis cultured in LES medium were collected,and then were inoculated into RPMI 1640 and modified PPMI 1640 medium according to the dose of 2 × 105respectively.Moreover,20% calf serum and antibiotics were added.The mediums were adjusted into pH 7.5,and then were deoxidized and cultured at 37 ℃ anaerobic jar.The morphology and growth summit of Blastocystis hominis were observed,and its proliferation numbers were counted every day.Results: The pH of modified RPMI1640 medium were constant at 7.5 within 10 days.The trophozoites of Blastocystis hominis in the modified RPMI1640 were 4 ~ 8μm in diameter with regular and clear morphologic structure.The most common form was the vacuolar,and the grnular form was common.The cysts seen in modified RPMI1640 medium was less than in RPMI 1640 medium.The growth fastigium of Blastocystis hominis was in the 4 ~ 5th day,and growth cycle was similar.But the numbers of Blastocystis hominis in modified RPMI1640 medium decreased slower than that in RPMI1640 medium when they reached the proliferation summit.Conclusion: The modified RPMI 1640 medium is suitable for Blastocystis hominis proliferation in vitro.