Effects of NF-κB p65 antisense oligodeoxynucleotide on high glucose-induced expression of extracellular matrix in proximal tublar epithelial cells

To explore the role of NF-κB in the renal interstitial fibrillation with diabetic nephropathy,we investigated the effects of nuclear factor of kappaB p65 (NF-κB p65) antisense oligodeoxynucleotide (AS-ODN) on high glucose induced expression of extracellular matrix (ECMs) in human proximal tubular epithelial cells (HK-2).We divided HK-2 cells into different groups with different concentrations of glucose (NG:5.5 mmol/L D-glucose;HG:30 mmol/L D-glucose;MG:5.5 mmol/L D-glucose +24.5 mmol/L mannitol),and different concentrations of AS-ODN/SofastTM (ODNL:1.2 μg/ml NF-κB p65 AS-ODN+30 mmol/L D-glucose;ODNH:1.8 μg/ml NF-κB p65 AS-ODN+30 mmol/L D-glucose) was transfected into HK-2 in HG group.We measured NF-κB protein expression in HK-2 cells by immunohistochemistry and detected the concentration of Fn and Col Ⅲ protein in supernatant of HK-2 cells by ELISA.In the result we found that the expression of NF-κB p65 protein in HG group increased significantly compared with that in NG group (P0.01),and the concentration of Fn and Col Ⅲ in HG group significantly higher than those in NG group (P0.01).But the NF-κB p65 protein expression of HK-2 cells in ODNL and ODNH groups with transfected NF-κB p65 AS-ODN decreased significantly in comparison with that in HG group without ones.The level of Fn and col Ⅲ in supernatant of cultured HK-2 cells in ODNL and ODNH groups were also significantly lower than that in HG group (P0.05 or P0.01).These results show that NF-κB AS-ODN can inhibit the expression of FN and Col Ⅲ in proximal tubular epithelial cells induced by high glucose,which may be one of the measures to prevent from fibrillation of kidney with diabetic nephropathy.