The Effects of All-trans Retinoic Acid on the Expression of Fibronectin and Laminin in Renal Tubular Epithelial Cells

Objective To investigate the effects of all-trans retinoic acid on the expression of Fibronectin(FN) and Laminin(LN) in human renal tubular epithelial cells(HK-2) so as to explore the role of all-trans retinoic acid on the pathogenesis of delaying renal interstitial fibrosis.Methods HK-2 cells cultured in vitro were divided randomly into eight groups:normal glucose group with 5.5 mM D-glucose(NG);high glucose group with 30 mM D-glucose(HG);mannitol group with 5.5 mM D-glucose and 24.5 mM mannitol(MG);all trans retinoic acid group with low concentrations(HG + 10-7 mol/LATRA,ATRA1);all trans retinioc acid group with medium concentrations(HG + 10-6 mol/LATRA,ATRA2);all trans retinioc acid group with high concentrations(HG + 10-5 mol/LATRA,ATRA3);ethanol group with HG + 10-2 mol/L ethanol and Captopril group with HG + 10-5 mol/Lcaptopril.Cells in each group were cultured for 24 h,48 h and 72 h respectively.The concentration of FN and LN in the culture medium was detected by enzyme linked immunosorbent assay(ELISA).Results High glucose could promote the expression of FN and LN in HK-2 cells(P 0.01),which could be inhibited by ATRA(P 0.01).Conclusion ATRA can inhibit the expression of FN and LN in HK-2 cells induced by high blood glucose.The inhibitory effect may be one of the potential measures to prevent from renal fibrillation with diabetic nephropathy.