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COMPARATIVE STUDY OF EXTRACTION METHOD FOR GENOMIC DNA IN MAT RUSH

Zhuoyi Wang

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Abstract

Seedlings of sixteen mat rush varieties planted in the field,and shoots derived from tissue culture of four varieties were used to investigate the effects of modified CTAB method and Isolation Kit method on the extraction quality of genomic DNA.The results showed that A_260nm and OD values of DNA solution of same material extracted by modified CTAB method were lower than those by isolation kit method.The concentrations of DNA solution were 0.65-4.90 μg/ml with the former method and 5.10-32.80 μg/ml with the latter method.Therefore,it was demonstrated that DNA Isolation Kit method was more suitable for genomic DNA extraction of mat rush.In addition,it was also found that extraction material had the significant impact on the extraction quality of genomic DNA,and the shoots developed by tissue culture were better than seedlings planted in the field.

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What this paper is about

Seedlings of sixteen mat rush varieties planted in the field,and shoots derived from tissue culture of four varieties were used to investigate the effects of modified CTAB method and Isolation Kit method on the extraction quality of genomic DNA.The results showed that A_260nm and OD values of DNA solution of same material extracted by modified CTAB method were lower than those by isolation kit method.The concentrations of DNA solution were 0.65-4.90 μg/ml with the former method and 5.10-32.80 μg/ml with the latter method.Therefore,it was demonstrated that DNA Isolation Kit method was more suitable for genomic DNA extraction of mat rush.In addition,it was also found that extraction material had the significant impact on the extraction quality of genomic DNA,and the shoots developed by tissue culture were better than seedlings planted in the field.

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Available abstract

Seedlings of sixteen mat rush varieties planted in the field,and shoots derived from tissue culture of four varieties were used to investigate the effects of modified CTAB method and Isolation Kit method on the extraction quality of genomic DNA.The results showed that A_260nm and OD values of DNA solution of same material extracted by modified CTAB method were lower than those by isolation kit method.The concentrations of DNA solution were 0.65-4.90 μg/ml with the former method and 5.10-32.80 μg/ml with the latter method.Therefore,it was demonstrated that DNA Isolation Kit method was more suitable for genomic DNA extraction of mat rush.In addition,it was also found that extraction material had the significant impact on the extraction quality of genomic DNA,and the shoots developed by tissue culture were better than seedlings planted in the field.

Key concepts: genomic DNA, DNA extraction, DNA, Extraction (chemistry), Shoot, Isolation (microbiology), Biology, Botany

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