2007•Chinese journal of integrated traditional and Western medicineRequires access
Effect of Yinxingye (银杏叶) on Bcl-2 mRNA and Bax mRNA expression following cerebral ischemia
Meng Yin
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Abstract
Objective: To explore whether expressions of Bcl-2 mRNA and Bax mRNA in the hippo-campus induced after ischemia can be modulated by chemistry pretreatment.Methods: The rat cerebral ischemia models were made by middle cerebral artery occlusion(MCAO) using improvement methods introduced by Nagasaway and Zea Longa.The rats were pretreated with Yinxingye(银杏叶) for 7 days before ischemian in Yinxingye pretreatment group.The changes of Bcl-2 mRNA and Bax mRNA expressions in the hippocampus of rats were measured by reverse transcription-polymerase chain reaction(RT-PCR) method in shame operation group,model group and Yinxingye pretreatment group at 6,12,24 and 48 hours after ischemia.Results: After MCAO,Bcl-2 mRNA and Bax mRNA were both induced to express.Level of Bcl-2 mRNA persistently increased at each time point,while the level of Bax mRNA increased gradually,reached a peak at 24 hours,and then decreased slowly.In comparison with the model group,after Yinxingye pretreatment for 7 days,Bcl-2 mRNA in the hippocampus was up-regulated at 6 and 24 hours after ischemia while Bax mRNA was down-regulated at 24 hours after ischemia.Conclusion: Yinxingye can efficiently regulate Bcl-2 mRNA and Bax mRNA expressions in hippocampus to protect the nerves after cerebral ischemic injury of rats.