Study on Genetic Diversity of Medicago falcata L. Germplasms

Taking four strains(1,2,3,4) and five germplasms(5,6,7,8,9)of Medicago falcata L.as materials,using 10%gel and SDS-PAGE electrophoresis,the seed storage proteins of individuals were studied,and the genetic diversity and their inter-relationship were analyzed.The results showed that the total dissoluble protein bands of the nine materials were 69,there were 56 polymorphism bands,the ratio of polymorphism bands was 81.16%,there were plenty genetic diversity at protein level in the nine germplasms of Medicago falcata L.The diversity order were: 861 5 4 3 2 9 7.Total of five germplasms had higher genetic diversity than four strains.The total Nei's gene diversity index was 0.2214,and the total genetic differentiation coefficient was 0.4929.Genetic differentiation coefficient of the four strains was higher than the five germplasms.Based on the Nei's genetic distance and using UPGMA method to cluster,the nine genetic resources were clustered into four groups,but the genetic relationship was different from the result obtained with mixed seeds samples.