2014•International Journal of Biosciences (IJB)Open access

Assessment of genetic diversity by seed storage proteins in wheat germplasms

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Abstract

The wealth of genetic diversity in 15 wheat (Triticum aestivum L.) local cultivars collected from various areas of Bajaur agency, Pakistan was investigated through biochemical characterization (SDS-PAGE). For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide gel Electrophoresis was carried out. The seed proteins were resolved on 12.25% polyacrylamide gel. Total 7 bands were observed in all fifteen genotypes. Among which 4 bands were monomorphic while 3 bands were polymorphic. Except for some minor differences in the intensity of the bands, major differences were not recorded in the protein profiles. Maximum 44% genetic diversity was observed in the collected lines. The linkage distances ranging from 00 to 44%. Cluster analysis exhibited moderate level of genetic diversity.

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The wealth of genetic diversity in 15 wheat (Triticum aestivum L.) local cultivars collected from various areas of Bajaur agency, Pakistan was investigated through biochemical characterization (SDS-PAGE). For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide gel Electrophoresis was carried out. The seed proteins were resolved on 12.25% polyacrylamide gel. Total 7 bands were observed in all fifteen genotypes. Among which 4 bands were monomorphic while 3 bands were polymorphic. Except for some minor differences in the intensity of the bands, major differences were not recorded in the protein profiles. Maximum 44% genetic diversity was observed in the collected lines. The linkage distances ranging from 00 to 44%. Cluster analysis exhibited moderate level of genetic diversity.

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Available abstract

The wealth of genetic diversity in 15 wheat (Triticum aestivum L.) local cultivars collected from various areas of Bajaur agency, Pakistan was investigated through biochemical characterization (SDS-PAGE). For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide gel Electrophoresis was carried out. The seed proteins were resolved on 12.25% polyacrylamide gel. Total 7 bands were observed in all fifteen genotypes. Among which 4 bands were monomorphic while 3 bands were polymorphic. Except for some minor differences in the intensity of the bands, major differences were not recorded in the protein profiles. Maximum 44% genetic diversity was observed in the collected lines. The linkage distances ranging from 00 to 44%. Cluster analysis exhibited moderate level of genetic diversity.

Key concepts: Storage protein, Genetic diversity, Germplasm, Biology, Agronomy, Biotechnology, Diversity (politics), Botany

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