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2004•Journal of Dalian UniversityRequires access

Study of the effect As_2O_3 on K_(562 )cells' apoptosis

Xiaodong Guan

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Abstract

[Objective] To observe the effect of As_2O_3 on inducing apoptosis of K_(562)cells. [Methods] K_(562)cells, which is erythroteckemin cell lines, were treated with As_2O_3 of 0,0.2,0.5,1.0,5.0 umol/L in concentration of 24,48,72 hours respectively, then harvested and detected with Hoechst 33342 staining as showing apoptosis cells, PI staining as showing necrosis cells and teypan blue staining as showing living cells.[Results]As_2O_3 inhibited significantly K_(562) cells growth compared with negative control(P0.01).Its inhibiting ratio was positive relation with the concentration of As_2O_3 and with time. [Conclusion] As_2O_3 is able to induce K_(562) cells into apoptosis.

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What this paper is about

[Objective] To observe the effect of As_2O_3 on inducing apoptosis of K_(562)cells. [Methods] K_(562)cells, which is erythroteckemin cell lines, were treated with As_2O_3 of 0,0.2,0.5,1.0,5.0 umol/L in concentration of 24,48,72 hours respectively, then harvested and detected with Hoechst 33342 staining as showing apoptosis cells, PI staining as showing necrosis cells and teypan blue staining as showing living cells.[Results]As_2O_3 inhibited significantly K_(562) cells growth compared with negative control(P0.01).Its inhibiting ratio was positive relation with the concentration of As_2O_3 and with time. [Conclusion] As_2O_3 is able to induce K_(562) cells into apoptosis.

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Available abstract

[Objective] To observe the effect of As_2O_3 on inducing apoptosis of K_(562)cells. [Methods] K_(562)cells, which is erythroteckemin cell lines, were treated with As_2O_3 of 0,0.2,0.5,1.0,5.0 umol/L in concentration of 24,48,72 hours respectively, then harvested and detected with Hoechst 33342 staining as showing apoptosis cells, PI staining as showing necrosis cells and teypan blue staining as showing living cells.[Results]As_2O_3 inhibited significantly K_(562) cells growth compared with negative control(P0.01).Its inhibiting ratio was positive relation with the concentration of As_2O_3 and with time. [Conclusion] As_2O_3 is able to induce K_(562) cells into apoptosis.

Key concepts: Apoptosis, Staining, Pi, Necrosis, Molecular biology, Chemistry, Cell culture, Positive control

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