Research on the Optimization of the Medium for Bacillus licheniformis Fermentation

[Objective] On the basis of the optimization of the Bacillus licheniformis fermentation medium,the best culture condition was determined for the reference for the implementation of its industrial production.[Method] On the basis of the results from Gu Chuntao in the liquid culture fermentation condition of Bacillus licheniformis-TS-1,the further test of the culture medium was conducted,and the experiment in the medium improvement for bacillus licheniformis was done with the single-factor orthogonal test with 4 factors and 3 levels for the optimum temperature,pH value and age of inoculation.[Results] The order of four kinds of factors significantly impacting the fermentation of Bacillus licheniformis was as follows: corn juice steep liquor from bean cake powder peptone glucose,and the best contain in the medium was 0.45 g corn steep liquor,1.50 g peptone,the ratio of steep liquor from bean cake powder in the medium of 1∶15 and 1.50 g glucose.The most appropriate inoculation age was 18 hours;inoculation amount,8%;the initial pH value,7.5 and the best temperature,37.5 ℃;under the condition,the logarithmic growth time of bacillus licheniformis appeared from the 14th to 20th hour,and the fermentation period was 22-24 hours.The number of viable cells per unit was raised to near 100 million cells and the cycle of the fermentation was shortened for more than 10 hours.[Conclusion] The best Bacillus licheniformis fermentation medium and culture condition were obtained,under the condition,the harvested biomass was greatly improved and the production cost was reduced,which was more conducive to its large-scale industrial production.