Relationship of Haplotypes of Four Polymorphisms in Fibrinogen Bβ-Chain with its Functional Expression and Cerebral Infarction

Objective To investigate the impact of Fbg-Bβ 1689T/G,16I/D,345C/T and Hinf 1 A/C and their haplotypes on functional expression of plasma fibrinogen(Fbg) concentration and molecular reactivity,and the relationship of them with cerebral infarction(CI).Methods The genotype and allele frequencies of four sites were analyzed by polymerase chain reaction-restriction fragment length polymorphisms(PCR-RFLP) and sequencing in 96 CI patients and 107 healthy controls.Fbg concentration,FMPV,Amax,FMPV/Amax and glucose were measured.Results There was no difference in genotype and allele frequencies of four locis between CI group and control(P0.05);FMPV and Amax in patients with CI was significantly higher than that of control(P0.01).In control,FMPV and Amax in patients with AC+CC of Hinf 1 was higher than that with AA(P0.05).Amax with TG+GG of 1689 in CI group was higher than that with TT(P0.05).Between CI group and control,no difference was found in haplotype frequencies of H1,H4,H5 and H7(χ2=1.95,P0.05).Conclusion In four sites,only variation genotype of Hinf 1 in 3' region can promot expression of FMPV and Amax,and Ones with variation genotype of Hinf 1 may be inclined to have much too risk of CI onset.There was no direct link between CI and major haplotypes of H1,H4,H5 and H7 composed of four polymorphisms,but they can influence functional expression of plasma fibrinogen concentration.