The relationship of multi-locus gene polymorphisms,functional expression of fibrinogen Bβ-chain and the type of cerebral infarction

Objective:To study the correlation of β-fibrinogen-854G/A,-455G/A,-249C/T,-148C/T,448G/A and Bcl-1G/A polymorphisms,functional expression of plasma fibrinogen concentration,molecular reactivity,and the type of cerebral infarction.Methods:A case-control study was used to analyze 54 patients with main-trunk cerebral infarction(MCI),106 patients with penetrating-arterial cerebral infarction(PCI) and 160 healthy cases as control group in Kailuan Hospital between July 2002 and June 2003.Gene polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Fg concentration,fibrin monomer polymerized velocity(FMPV),absorbance maximum(Amax),FMPV/Amax and biochemistry factors including TG were measured.Results:Fg concentration,FMPV,FMPV/Amax in the MCI group and TG,VLDL and FMPV in the PCI group were higher than in the control group(P0.05).The frequencies of-854A and Bcl-1A alleles had significant difference among three groups,and the frequencies of GA and AA genotypes in the MCI and PCI groups were higher than in the control group(P0.05),however,no different genotypes and allele frequencies of the remaining sites were found in the three groups(P0.05).Fg concentration and FMPV of allele T carriers in the MCI group were less than that of-249C/C homozygous ones(P0.05);FMPV/Amax of allele T carriers in the PCI group was higher than that of-148C/C homozygous ones(P0.05);with allele A carriers,Fg concentration of control group and FMPV of PCI group were higher than that of Bcl-1 wild homozygote(P0.05).Conclusion:Bβ-249 C/T polymorphism in the 5-flanking promoter region can influence the expression of plasma FMPV,Bβ-148 locus is the main regulation location of Fg molecular conglomerate function.Bcl-1 locus in the 3-flanking region is an important gene regulator of plasma Fg concentration,moreover,people with its mutated genotypes are susceptible to MCI.The abnormal plasma Fg concentration,FMPV/Amax and FMPV simultaneously are important risk factors for MCI,and only abnormal FMPV and TG are prone to PCI.