THE mRNA EXPRESSION OF GENE P IN HBV REVERSE TRANSCIPTASE OF PBMCs,THE INTEGRATION OF GENE X AND THEIR RELATIONS TO PRIMARY HEPATOMA

Objective To explore the expression of mRNA of gen P in HBV polymerase/reverse transcriptase of peripheral blood mononuclear cells(PBMCs),the integration of gene X and their relations to hepatoma.Methods Primers P_1 and P_2 were designed for HBV gene P,and X_1 and X_2 for genex.PMBCs were isolated from 40 HBsAg-positive cases of primary hepatoma and 36 cases of chronic hepatitis B(HB).Total DNA and total RNA in genome were extracted.RNA was made into cDNA after reverse synthesis.The integrations of HBV gene X were detected through PCR.mRNA expressions of gene P in PBMCs in HBV were detected through RT-PCR method.Results The integration rate of gene X in PBMCs of the primary hepatoma(PH) group and the chronic HV(CHV) group were 79.5%(31/40) and 50%(18/36) respectively(P0.05).The mRNA expression rates of gene P in the former group and the latter group were 10%(4/40) and 33.3%(12/36) respectively(P0.05).Conclusion HBV replications remain relatively resting in the primary hepatoma group,and the intergration of a great number of gene X leads to the occurrence of the disease.