Effect of celecoxib on anti-proliferation and Inducing apoptosis of human osteosarcoma cell MG-63 line

Objective:To study and detect the effect of celecoxib on anti-proliferation and inducing apoptosis of human osteosarcoma cell MG-63 line in vitro.Method:Growth suppression was detected by MTT method.Special changes of apoptosis in morphology were observed by fluorescence microscopy,transmission electron microscopy (TEM) and TDT-mediated dUTP-biotin nick end-labeling (TUNEL) assay.The apoptosis rates were quantified by flow cytometry (FCM).Result:The growth inhibition rate of MG-63 cells treated with 10,20,40,80μmol/l celecoxib were 5.16%,16.53%,48.31%,87.76% respectively(P0.05,P0.01).Through TEM and TUNEL assay,the special morphological changes were observed,such as nuclear chromatin condensation,fragmentation,cell shrinkage,and the formation of apoptotic bodies.At different time of 24,48 and 72 hours,the apoptotic rates of MG-63 cells treated with celecoxib 40μmol/l were respectively,significantly higher than that of the control group (P0.01).Conclusion:Celecoxib could inhibit the proliferation of human osteosarcoma cell MG-63 line and induce the cell apoptosis.It is a very interesting subject to further investigate the mechanism.